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. 2015 Dec 18;11(2):467–475. doi: 10.3892/etm.2015.2953

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Growth of mesenchymal stem cells (MSCs) and pulmonary artery smooth muscle cells (PASMCs) in vitro. (A) MSCs culured in serum-free medium at passage 3 (magnification, ×100). (B) Osteogenic and (C) adipogenic differentiation of MSCs cultured in MSC-conditioned media identified using Alizarin red S or Oil Red O stain, respectively (magnification, ×200). (D) Phenotypic analysis of MSCs, assessed using flow cytometry. (E) PASMC tissue explant cultured for 5 days (magnification, ×100). (F) PASMCs at passage 3 (magnification, ×100). (G) PASMC immunofluorescent staining for α-smooth muscle actin (magnification, ×200). CD, cluster of differentiation; Sox-2, SRY box-2; HLA, human leukocyte antigen.