Figure 3.
HCV NS5A protein levels in Huh7 HCV replicon cells treated with GA. Huh7 HCV replicon cells (6×105 cells) were incubated in the presence (lanes 5–7) or absence (lanes 1–4) of 300 µM GA for different times (0, 24, 48, and 72 h). (A) Cell lysates were prepared, and equal amounts of protein extracts (50 µg) were subjected to immunoblot analysis to detect NS5A (top panel) and actin levels (bottom panel) by western blot analysis. (B) Quantified ratios of NS5A to actin proteins from the immunoblot detection. *P<0.05 vs. control. HCV, hepatitis C virus; NS5A, nonstructural protein 5A; GA, gallic acid.