Figure 4.

Effect of GA on intracellular ROS levels in Huh7 HCV replicon cells. HCV replicon-containing cells (2×10⁴ cells) were incubated with or without 300 µM GA for different times. Next, ROS levels were assessed in total cellular extracts using a 2′,7′-dichlorofluorescein diacetate fluorescence assay and the fluorescence was measured at 485 nm (excitation) and 528 nm (emission). PDTC (5 µM) was used as a control due to its antioxidant effect and H₂O₂ (2 µM) was used as a positive control of ROS production, and were treated similar to the GA treatment. Data are expressed as the mean ± standard deviation of triplicate cultures, and the experiment was repeated 3 times (*P<0.05). GA, gallic acid; ROS, reactive oxygen species; HCV, hepatitis C virus; PDTC, pyrrolidine dithiocarbamate.