FOXC1 expression in serous ovarian carcinoma cell lines. (A) FOXC1 mRNA levels in SKOV-3 and HO-8910 cell lines. (B) FOXC1 protein levels in SKOV-3 and HO-8910 cell lines. Ns and Nh represent nuclear protein of SKOV-3 and HO-8910 cells, respectively; Cs and Ch represent cytoplasmic protein of SKOV-3 and HO-8910 cells, respectively. β-actin was used as an internal control in western blot analysis. (C) Left panel: Nuclear and cytoplasmic immunostaining of FOXC1 was observed in SKOV-3 cells, although staining was less intense in the cytoplasm than in the nucleus, which was in accordance with the results of western blot analysis. Right panel: Nuclear and cytoplasmic staining of FOXC1 was observed in HO-8910 cells, although staining was less intense in the cytoplasm than in the nucleus, which was in accordance with the results of western blot analysis.