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. 2015 Dec 9;11(2):1242–1250. doi: 10.3892/ol.2015.4018

Figure 1.

Figure 1.

Combined treatment with Lv/sh-Stat3 and Lv/sh-ADAM9 inhibits the expression of Stat3 and ADAM9 in A549 cells. Reverse transcription-quantitative polymerase chain reaction analysis of the messenger RNA expression levels of (A) Stat3 and (B) ADAM9 in A549 cells infected with Lv/sh-Stat3 and Lv/sh-ADAM9, alone or in combination. (C) Western blot analysis of Stat3 and ADAM9 in A549 cells infected with Lv/sh-Stat3 and Lv/sh-ADAM9, alone or in combination, using specific antibodies. β-actin served as internal control. (D) Relative quantification of the protein levels of Stat3 and ADAM9 in A549 cells by densitometric analysis. *P<0.05 vs. control. Lv, lentiviral; sh, small hairpin; Stat3, signal transducer and activator of transcription 3; ADAM9, disintegrin and metalloproteinase 9.