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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochemistry. 2015 Nov 30;54(49):7157–7167. doi: 10.1021/acs.biochem.5b01065

Figure 2. EPR line shape depends on choice of membrane mimic.

Figure 2

Normalized X-band CW EPR spectra of M2TMC (23–60) spin labeled at I42C in DHPC micelles, POPC/POPG (4:1) bilayers with varying molar quantities of cholesterol, and a viral membrane mimic (SM:DPPC:DPPE:Chol:PEG-PE 23:21:21:30:1). Each sample was spin-diluted with unlabeled peptide (unlabeled:labeled 4:1) to eliminate broadening resulting from spin-coupling between monomers within the homotetrameric protein. The darker gray box indicates a broader, more immobilized component (I) that increases in intensity from top to bottom. The more mobile component (M) indicated by a lighter gray box decreases in intensity from top to bottom.