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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochemistry. 2015 Nov 30;54(49):7157–7167. doi: 10.1021/acs.biochem.5b01065

Figure 7. DEER reveals presence of multiple distances and protein conformations.

Figure 7

Tikhonov Regularization and distance distributions of M2TMC-I51C (λ=40, top) and M2TMC-F55C (λ=25, bottom). Background-subtracted DEER data are shown as open circles and corresponding fits as black lines. L-curves are shown in Figure S4. M2 peptides were incorporated into1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) at a peptide:DHPC molar ratio of 1:500.