Table 2.

Basic Setting Parameters in the Dispensing Programs and Spotting

Tip setting
Aspiration volume (µl)	3
Aspiration flow rate (µl/sec)	0.20
Return sample in source plate after spotting	No
Return sample in source plate if failed stroboscope check	No
Delay time (sec)	1.0
Before aspiration dry pad time (sec)	0.10
after aspiration dry pad time (sec)	0.0
Piezo voltage (V)	100
Piezo pulse width (µsec)	50
Piezo frequency (100 Hz)	100
Wash conditions
Wash time	30.00
Dry time (sec)	0.10
Extrawash well	Extrawash
Extrawash volume	0.00
Extrawash flow rate (µl/sec)	0.50
Extrawash piezo	No
Stroboscope details
Delay before check	0.50
Checks	4
Delay between checks (sec)	0.10
% CV tolerance across checks	5%
Z heights
Z height from target	0.3
Z height from traverse target	0.0