Figure 1.

Characteristics of MKN-45 human gastric cancer cells and KRC-108-resistant clones (MKN-R1, -R2 and -R3). (A) MKN-45, MKN-R1, MKN-R2 and MKN-R3 cells were treated with KRC-108 at the indicated concentrations for 72 h before subjection to a cell viability assay. Data are presented as the means from three independent experiments and bars represent standard error. *P<0.05 vs. DMSO control. (B) Cells were seeded into a 24-well plate, and the cell numbers were counted each day until day 4 to establish a growth curve. Data are presented as the mean and standard error of three independent experiments. *P<0.05 vs. MKN-45 cells (C) The expression levels of c-Met and p-Met following KRC-108 treatment were measured by western blotting in MKN-45 and MKN-R cells. (D) The expression of c-Met and the phosphorylation of c-Met were analyzed by immunofluorescence (magnification, ×630). Red, c-Met (top row) or p-Met (bottom row); blue, DAPI. p-Met, phosphorylated c-Met.