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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Mol Immunol. 2015 Jul 19;67(2 0 0):317–324. doi: 10.1016/j.molimm.2015.06.015

Figure 2. Cdk5 activity neither alters Smad activation nor binding to the Foxp3 gene.

Figure 2

A) Naïve primary T cells were stimulated with anti-CD3/CD28, with or without TGF-β, IL-6 and CIP for 72hrs. Whole cell protein lysates were prepared from these samples and western blot analyses were performed to probe for p-Smad2 (Ser465/467), p-Smad3 (Ser423/425) and total Smad2 and total Smad3. B) Similarly, both Cdk5+/+ T cells and Cdk5−/− T cells were stimulated and treated under conditions noted in (A) and probed for the presence of p-Smad2/3 and total Smad2/3. C) Luciferase activity was examined in EL4 T cells transfected with the Smad-binding element (SBE) reporter construct and E) the Foxp3 promoter and enhancer I reporter construct. Cells were stimulated with anti-CD3/CD28 with or without TGF-β, IL-6 and CIP as indicated.