Figure 3.

Widespread changes in splicing events after PNN knockdown. A scatterplot of splicing events detected in pinin (PNN) knockdown and control cells (the overlapping area in the Venn diagrams), plotting the mean expression (as log₂ APN) of the control cells versus the PNN-knockdown cells. Differentially expressed events are red (upregulated) and blue (downregulated), and non-differentially expressed events are in black. More than 70% of significantly different events are upregulated in PNN (p<0.0001). All types of events are biased toward upregulation in PNN (92.5% of all events, 91.8% of exon-skipping events (635 of 692 differentially expressed [DE] events), 94.1% of alternative donor sites (531 of 564 DE events), 93.0% of alternative receptor sites (490 of 527 DE events), 93.7% of events involving alternative donors and acceptors (554 of 591 DE events), and 62.9% of intron retention events (95 of 151 DE events).