Table 1.
Foc proteins identified in the xylem of Foc-infected B. oleracea cv. Delicious.
| No. | Accession numbera | Descriptionb | Mass (kDa) | SPc | Length (aa)d | Cys Number | Score/Coveragee |
|---|---|---|---|---|---|---|---|
| 1 | P00041/contig_75_2 | Carboxylesterase, type B | 60.9 | N | 558 | 5(0.90%) | 821/43.4 |
| 2 | P01134/contig_644_31 | Ferric reductase Fre2p | 104.8 | Y | 932 | 16(1.72%) | 305/5.6 |
| 3 | P01592/contig_663_2 | Putative glycosidase crf1 | 29.3 | Y | 274 | 5(1.82%) | 350/19 |
| 4 | P03403/contig_739_59 | Protein SnodProt1 | 14.6 | Y | 139 | 4(2.88%) | 636/70.5 |
| 5 | P04292/contig_761_13 | Endoglucanase c | 32.3 | Y | 294 | 3(1.02%) | 228/26.5 |
| 6 | P05132/contig_785_69 | Valacyclovir hydrolase | 32.0 | N | 290 | 1(0.34%) | 397/39.7 |
| 7 | P08654/contig_907_28 | Alcohol dehydrogenase | 65.3 | Y/M | 609 | 3(0.49%) | 235/20.9 |
| 8 | P10310/contig_989_8 | Putative serine-rich protein | 41.3 | Y | 388 | 8(2.06%) | 116/5.7 |
| 9 | P10456/contig_995_21 | Endo-1,4-beta-xylanase C | 35.7 | Y | 328 | 2(0.61%) | 211/23.2 |
| 10 | P11164/contig_1045_2 | Chitinase | 47.4 | Y | 432 | 13(3.01%) | 130/19.9 |
| 11 | P11220/contig_1051_3 | BNR/Asp-box repeat domain protein | 41.3 | Y | 379 | 3(0.79%) | 1045/52.0 |
| 12 | P11242/contig_1054_1 | Hypothetical protein | 12.4 | Y | 117 | 2(1.71%) | 259/50.4 |
| 13 | P11311/contig_1060_12 | LysM domain-containing protein | 28.4 | Y/M | 265 | 12(4.53%) | 167/11.7 |
| 14 | P11347/contig_1063_1 | Secreted oxidoreductase ORX1-like protein | 65.2 | Y | 610 | 3(0.49%) | 688/37.1 |
| 15 | P13298/contig_1456_1 | Hypothetical protein | 19.1 | Y | 170 | 2(1.18%) | 217/40.6 |
| 16 | P13299/contig_1456_2 | Hypothetical protein | 25.2 | N | 228 | 10(4.39%) | 927/41.7 |
| 17 | P13310/contig_1464_1 | Hatching enzyme | 38.2 | Y | 338 | 6(1.78%) | 511/47.9 |
| 18 | P13373/contig_1509_1 | Hypothetical protein | 29.1 | Y | 263 | 8(3.04%) | 464/38.0 |
| 19 | P14728/contig_2465_1 | Foc-SIX4 | 27.3 | Y | 252 | 6(2.38%) | 296/35.3 |
| 20 | P14743/contig_2555_1 | Hatching enzyme | 45.3 | Y | 402 | 12(2.99%) | 567/46.5 |
| 21 | P15123/contig_3892_1 | FAD binding domain-containing protein | 55.0 | Y/M | 497 | 2(0.40%) | 328/19.9 |
| 22 | P15632/contig_3990_11 | Chitinase A1 | 45.6 | Y | 419 | 5(1.19%) | 563/28.6 |
| 23 | P15981/contig_4126_1 | Foc-SIX1 | 30.8 | Y | 279 | 9(3.23%) | 369/52.7 |
| 24 | P16246/contig_4338_1 | Endo-1,4-beta-xylanase | 25.1 | Y | 232 | 0(0.00%) | 202/32.3 |
| 25 | P16923/contig_5980_1 | hypothetical protein | 18.6 | Y | 167 | 2(1.20%) | 241/74.9 |
Serial number/contig number of Foc Cong: 1-1 genome database used in this study. The numbers given in bold indicate a small (less than 300 amino acids) cysteine-contained secreted protein.
Genes were annotated by NCBI blastp. Descriptions of the functional/putative functional protein item were used. Descriptions given in bold were discussed in Discussion. A detail result of protein BLAST consults Table S4.
SP indicates a predicted signal peptide denoting secretion. The signal peptide was predicted using SignalP (http://www.cbs.dtu.dk/services/SignalP/) and TargetP (http://www.cbs.dtu.dk/services/TargetP/). “Y” and “N” indicate whether or not the sequence contains a signal peptide in secretory pathway. When both predictions are consistent, only the SignalP result is shown. When it is not the case, both predictions are shown. “M” indicates that TargetP predicted a mitochondrial targeting peptide.
Length given in amino acid number is count from the full length of predicted peptide sequence generated from Foc genome information.
Number before the slash indicates the score and after the slash indicated the coverage (given in percentage) evaluated by MASCOT.