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Reverse transcription-quantitiative polymerase chain reaction was performed twice, data sets 1 and 2, to confirm that stable cell lines of Foxc2 shRNA had been established. The percentages of HCT-116shFoxc2 are 0.5039 and 0.1928% in data sets 1 and 2, respectively. Foxc2, forkhead box C2 gene, 116-prs, human colon cancer HCT-116 cell line; 116-shFoxc2, human colon cancer HCT-116 cell line with short hairpin Foxc2 RNA.
