Figure 3.

HK II dissociation from mitochondria is increased in ErbB2-overexpressing breast cancer cells. (A) The intact mitochondrial fractions from 231V and 231ErbB2 cells and (B) BT474 scramble and BT474 siErbB2 cells were isolated and treated with 3-BrPA for 2 h. The fractions were then centrifuged to separate the supernatant and mitochondria. The amount of HK II in the supernatant was an indicator of the dissociation of mitochondria by 3-BrPA (left) and the western blots were quantified (right). AIF was used as a positive control and prohibitin as a negative control. Data are the mean values of three independent experiments; bars, SE. **P<0.01.