Table I.
Antibodies used for immunohistochemistry.
| Antigen | Clone | Dilution | Manufacturer | Staining results |
|---|---|---|---|---|
| Cytokeratin | AE1/AE3 | 1:1,500 | Dako | Negative |
| CDK-4 | DCS-31 | 1:4,000 | Invitrogen | Negative |
| CD30 | Ber-H2 | 1:100 | Dako | Positive |
| CD117 | NA | 1:450 | Dako | Negative |
| ALK | 5A4 | 1:50 | Abcam | Positive |
| β-catenin | β-catenin-1 | Ready-to-use | Dako | Negative |
| SMA | 1A4 | 1:200 | Dako | Positive |
All performed with high-voltage microwave boiling in citrate buffer pH 6.0 for 12.5 min for all antibodies as the antigen retrieval method. CDK, cyclin-dependent kinase; ALK, anaplastic lymphoma kinase; SMA, smooth muscle actin; NA, not available.