Figure 5. Cell survival as a function of pulse magnitude for different extracellular media.

Cloning efficiency tests were performed after exposure to one single micropulse of 100 μs. External medium always contained 30 nM bleomycin. Directly after the delivery of the pulse, cells were diluted in S-MEM medium containing 30 nM bleomycin. Results are represented as mean values + SD of three to six independent experiments. Cell survival is normalized on the control submitted to the bleomycin alone. Dead cells are the ones reversibly permeabilized since pulse treatment alone (i.e. without bleomycin) did not induce any cell death (data not shown).