Table 1. Composition and properties of the different media.
Buffer | Sugar | MgCl2 | NaCl | PH Buffer | σ [S/m] | osm [mOsm] |
---|---|---|---|---|---|---|
STM | Sucrose/50 | 1 | – | Tris/10 | 0.096 | 287 |
ATM | Adonitol/250 | 1 | – | Tris/10 | 0.093 | 280 |
GTM | Glucose/250 | 1 | – | Tris/10 | 0.106 | 274 |
SNM | Sucrose/250 | 1 | 5 | Na2HPO4/5 - NaH2PO4/5 | 0.092 | 274 |
SHM | Sucrose/250 | 1 | 6 | Hepes/10 | 0.092 | 284 |
S5HM | Sucrose/210 | 1 | 30 | Hepes/50 | 0.375 | 351 |
S5TM | Sucrose/210 | 1 | – | Tris/50 | 0.375 | 318 |
NSHM | Sucrose/110 | 1 | 74 | Hepes/10 | 0.600 | 275 |
NSTM | Sucrose/110 | 1 | 70 | Tris/10 | 0.600 | 283 |
NHM | – | 1 | 140 | Hepes/10 | 1.450 | 263 |
NTM | – | 1 | 140 | Tris/10 | 1.421 | 283 |
PBS | cf commercial technical ressource | 1.436 | 295 | |||
SMEM | cf commercial technical ressource | 1.413 | 288 |
Concentrations are given in mM. pH was adjusted to 7 by addition of HCl.