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Zeta potential (ζ) and hydrodynamic radii (RH) of PAA-coated PLGA-PEI nanoparticles in dependence on pH during PAA adsorption. Nanoparticle radii were obtained in ultrapure water (H2O) and 1 mM sodium hydroxide solution (NaOH), respectively. All experiments were performed in triplicate, average values ± standard deviations are shown.
| pH (PAA) | ζ/mV (H2O) | RH/nm (H2O) | RH/nm (NaOH) |
| 3 | −59 ± 1 | 130 ± 2 | 250 ± 2 |
| 5 | −40 ± 2 | 126 ± 1 | 142 ± 2 |
| 7 | −30 ± 6 | 126 ± 2 | 134 ± 5 |
