Table 2. Rotavirus genotyping on clinical samples using qRT-PCR assay and compared to RT-PCR and sequencing as gold standard.

Well 1A	RotaTeq® VP6 gene sequencing
RotaTeq® VP6 HEX qRT-PCR assay	Positive	Negative	Total
Positive	83	0	83
Negative	0	770	770
Total	83	770	853
Well 1B	Xeno or MS2 RNA spiked before extraction
Xeno or MS2 TR qRT-PCR assays	Positive	Negative	Total
Positive	729	0	729
Negative	0	124	124
Total	729	124	853
Well 1C	Rotarix® NSP2 gene sequencing
Rotarix® NSP2 Cy5 qRT-PCR assay	Positive	Negative	Total
Positive	39	0	39
Negative	0	814	853
Total	39	814	853
Well 1D	VP7-G12 gene sequencing
VP7-G12 FAM qRT-PCR assay	Positive	Negative	Total
Positive	173	0	173
Negative	2	678	680
Total	175	678	853
Well 2A	VP7-G9 gene sequencing
VP7-G9 HEX qRT-PCR assay	Positive	Negative	Total
Positive	109	2	111
Negative	1	741	742
Total	110	743	853
Well 2B	NSP3-TR RT-PCR
NSP3-TR qRT-PCR assay	Positive	Negative	Total
Positive	833	0	833
Negative	0	20	20
Total	833	20	853
Well 2C	VP7-G4 gene sequencing
VP7-G4-FAM qRT-PCR assay	Positive	Negative	Total
Positive	79	0	79
Negative	0	774	774
Total	79	774	853
Well 3A	VP7-G1 gene sequencing
VP7-G1 HEX qRT-PCR assay	Positive	Negative	Total
Positive	161	0	161
Negative	0	692	692
Total	161	692	853
Well 3B	VP4-P4 gene sequencing
VP4-P4 TR qRT-PCR assay	Positive	Negative	Total
Positive	103	0	103
Negative	0	750	750
Total	103	750	853
Well 3C	VP7-G3 gene sequencing
VP7-G3 Cy5 qRT-PCR assay	Positive	Negative	Total
Positive	143	0	143
Negative	1	709	710
Total	144	709	853
Well 4A	VP7-G2 gene sequencing
VP7-G2 TR qRT-PCR assay	Positive	Negative	Total
Positive	110	0	110
Negative	0	743	743
Total	110	743	853
Well 4B	VP4-P8 gene sequencing
VP4-P8 Cy5 qRT-PCR assay	Positive	Negative	Total
Positive	596	0	596
Negative	7	250	257
Total	603	250	853
Well 4C	VP4-P6 gene sequencing
VP4-P6 FAM qRT-PCR assay	Positive	Negative	Total
Positive	56	0	56
Negative	0	797	797
Total	56	797	853