Table 3. Performance characteristics of rotavirus genotyping qRT-PCR assay.

Multiplex qRT-PCR Wells	qRT-PCR assay	Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	Limit of detection (copies)		Efficiency (%)
						Singleplex	Multiplex	Singleplex	Multiplex
Well 1	RotaTeq®-VP6 HEX	100	100	100	100	1	10	102	93
	Xeno or MS2 TR	100	100	100	100	–	–	–	–
	Rotarix®-NSP2 Cy5	100	100	100	100	2	200	91	93
	VP7-G12 FAM	98.8	100	100	99.7	60	600	88	81
Well 2	VP7-G9 HEX	99	99.7	98.1	99.8	60	600	92	92
	NSP3 TR	100	100	100	100	2	20	93	96
	VP7-G4 FAM	100	100	100	100	15	150	90	89
Well 3	VP7-G1 HEX	100	100	100	100	5	500	95	92
	VP4-P[4] TR	100	100	100	100	12	120	88	82
	VP7-G3 Cy5	99.3	100	100	99.8	4	400	86	87
Well 4	VP7-G2 TR	100	100	100	100	4	400	85	92
	VP4-P[8] Cy5	98.8	100	100	97.2	30	300	89	82
	VP4-P[6] FAM	100	100	100	100	400	4000	86	90