Table 1. Primers used for screening of human-parasitizing tick specimens, Texas, USA, October 1, 2008–September 30, 2014.
| Primer name | Gene | Primer sequence, 5′ → 3′ | Specificity | Amplicon, bp | Reference |
|---|---|---|---|---|---|
| Borrelia spp. | |||||
| FlaLL | flaB | ACATATTCAGATGCAGACAGAGGT | Genus | 664 | (3) |
| FlaRL | flaB | GCAATCATAGCCATTGCAGATTGT | Genus | (3) | |
| FlaLS | flaB | AACAGCTGAAGAGCTTGGAATG | Genus | 330 | (3) |
| FlaRS | flaB | CTTTGATCACTTATCATTCTAATAGC | Genus | (3) | |
| BL-Fla 522F | flaB | GGTACATATTCAGATGCAGACAGAGGG | B. lonestari | 660 | (2) |
| BL-Fla 1182R | flaB | GCACTTGATTTGCTTGTGCAATCATAGCC | B. lonestari | (2) | |
| BL-Fla 662F | flaB | CTGAAGAGCTTGGAATGCAACCTGC | B. lonestari | 198 | (2) |
| BL-Fla 860R | flaB | GAGCTAATCCCACCTTGAGCTGG | B. lonestari | (2) | |
| BL-16S 227F | 16S | TCACACTGGAACTGAGATACGGTCC | Genus | 693 |
(2) |
| BL-16S 920R |
16S |
GAATTAAACCACATGCTCCACCGC |
Genus |
(2) |
|
| Rickettsia spp. | |||||
| Rr.190 70P | rompA | ATGGCGAATATTTCTCCAAAA | Genus | 532 | (4) |
| Rr.190 602N | rompA | AGTGCAGCATTCGCTCCCCCT | Genus | (4) | |
| BG1–21 | rompB | GGCAATTAATATCGCTGACGG | Genus | 650 |
(5) |
| BG2–20 |
rompB
|
GCATCTGCACTAGCACTTTC |
Genus |
(5) |
|
| Ehrlichia spp. | |||||
| Ehr DSB 330F | dsb | GATGATGTCTGAAGATATGAAACAAAT | Genus | 398 | (6) |
| Ehr DSB 728R | dsb | CTGCTCGTCTATTTTACTTCTTAAAGT | Genus | (6) | |
| Ehr map1F | map1 | ATTTTTACCTGGTGTGTCCTTTTCTGA | Genus | 873 |
(7) |
| Ehr map1R | map1 | CCTTCCTCCAATTTCTATACC | Genus | (7) | |
| Ehr Pmap2F | map1 | GACACCAAGGCAGTATACGG | Genus | (7) | |
| Ehr Pmap2R |
map1 |
CTAAGTCAGTACCAATACCTGCAC |
Genus |
(7) |
|
| Tick DNA | |||||
| 16S-1 | mt16S | CCGGTCTGAACTCAGATCAAG | Unknown | 300 | (8) |
| 16S+2 | mt16S | TTGGGCAAGAAGACCCTATGAA | Unknown | (8) | |