Figure 2.
Tissue- and cell compartment-specific expression of MTI1 and DEP1. A, B, D, and E, GUS staining of flowers (A1 and A3), unopened siliques (B1 and B3), rosette leaves (D1 and D3), 7-d-old seedlings (E2 and E5), cross sections of leave vascular bundles (D2 and D4), cross sections of root differentiation zones (E1 and E4), and cross sections of root tips (E3 and E6). A, B, C, F, and G, GFP fluorescence of flowers (A2 and A4), seeds (B2 and B4), embryos (C1 and C5, early globular stage and funiculus; C2 and C6, globular stage; C3 and C7, heart stage; C4 and C8, torpedo stage), roots (F1, F2, F4, and F5, root differentiation zones; F3 and F6, root tips) and Arabidopsis mesophyll protoplasts expressing C-terminal GFP fusions (G1–3, MTI1; G4–6, DEP1). All constructs were cotransformed with a cytosolic mCherry expression construct. G1 and G4, GFP fluorescence is shown in green. G2 and G5, mCherry fluorescence is shown in red. G3 and G6, Merged fluorescence is shown in yellow. Chloroplasts are shown in blue. Bars = 5 mm for D1 and D3, 2 mm for B1 and B3, 1 mm for A1 to A4, 200 µm for E2 and E5, 50 µm for B2, B4, and F1–6, 10 µm for C1, C4, C5, and C8, 5 µm for G1–6, and 1 µm for C2, C3, C6, C7, D2, D4, E1, E3, E4, and E6.