Figure 8.

Shoot Cl⁻ concentration was significantly reduced in the NPF2.4 amiRNA knockdowns under low salt conditions (2 mm NaCl), whereas the shoot NO₃^– concentration of the NPF2.4 knockdowns was similar to null segregants. Four-week-old hydroponically grown plants were treated with 2 mm NaCl for 5 d. A, NPF2.4 expression in the roots of NPF2.4 knockdowns. B, Cl^– concentrations in the shoot of NPF2.4 knockdowns and null segregants. C, Shoot NO₃^– concentrations of NPF2.4 knockdowns and null segregants. Results are presented as mean ± se, n = 4 to 7. Significance is indicated by the asterisks (one-way ANOVA and Tukey test, * P ≤ 0.05, **P ≤ 0.005, and ***P ≤ 0.001).