Figure 2.

Nucleocytoplasmic shuttling of SR34 and SR34a in Arabidopsis transgenics. FLIP-shuttling of SR34-GFP (A) and SR34a-GFP (B) was monitored in the absence (−LMB) and upon LMB (+LMB) treatment in root cells. One hundred percent fluorescence indicates prebleach fluorescence intensity. As a control, cells were repeatedly scanned under no photobleaching conditions and fluorescence was quantified. Half-time of fluorescence decay for –LMB and +LMB curves were as follows (in seconds): ∼ 38 and ∼ 80 (A), ∼ 32 and ∼ 78 (B), respectively. Values are means ± SEM for at least 16 nuclei. The curves show a significant inhibitory effect of LMB on shuttling (P < 0.0001).