Figure 3.

Nucleocytoplasmic shuttling of SRSF1 subfamily members in transient expression assays in tobacco leaf cells. FLIP-shuttling of SR30-GFP (A), SR34-GFP (B) and SR34a-GFP (C) was monitored in the absence (−LMB) and upon LMB (+LMB) treatment in leaf epidermal cells. One hundred percent fluorescence indicates prebleach fluorescence intensity. As a control, cells were repeatedly scanned under no-photobleaching conditions and fluorescence was quantified. Values are means ± SEM for at least 13 nuclei. The FLIP curves show a significant inhibitory effect of LMB on shuttling for SR34 and SR34a (P < 0.0001).