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. 2015 Dec 3;170(2):1046–1059. doi: 10.1104/pp.15.01407

Figure 1.

Figure 1.

Expression of CML38 and CML39 in hypoxia-stressed Arabidopsis seedlings. A, Q-PCR analysis of CML38 and CML39 expression in roots (squares) and shoots (circles) of hypoxia-challenged 2-week-old Arabidopsis seedlings. Normalized expression represents 2−ΔΔCt values, obtained by subtracting the threshold cycle value (Ct) of CML38 or CML39 from that of the internal reference gene, UBIQUITIN10 (UBQ10), and using the 0-h shoot sample as a calibrator. Error bars indicate se of three biological replicates. B, CML38::GUS expression in 10-d-old hypoxia-treated seedlings. Untreated negative control and hypoxia-treated (4, 8, and 12 h) seedlings are shown. The bottom images show magnifications of the root tip area. Bars = 500 µm (top) and 25 µm (bottom).