Figure 3.

Transcript and protein levels in hypoxia-challenged CML38-YFP recombineering plants. A, Time course of CML38-YFP transcript expression assayed by Q-PCR in the root tissue of 31-d-old hydroponically grown plants in response to hypoxia. Normalized expression was determined as described in Figure 1. Error bars indicate se of three technical replicates. B, Time course of CML38-YFP fusion protein (102.1 kD) expression assayed by western blot in the root tissue of 31-d-old hydroponically grown plants in response to hypoxia. Top gel, Western-blot chemiluminescent signal; bottom gel, Coomassie Blue-stained gel from the same extracts. Each lane contained 30 μg of protein. The electrophoretic mobility positions of the molecular mass markers are shown at left.