Figure 10.

PSII supercomplex accumulation is impaired in TEF30-amiRNA strains exposed to HL. cw15-325 control (Con) and TEF30-amiRNA cells were grown at 30 µmol photons m⁻² s⁻¹ (LL) and exposed to 800 µmol photons m⁻² s⁻¹ (HL) for 4 h. Thylakoid membranes were isolated and solubilized with 1% α-DDM, and protein complexes were separated electrophoretically on a 7.5% native Deriphat-polyacrylamide gel. The gel was then photographed (left) and processed for immunoblotting using antibodies against TEF30 and the D1 protein (right). Bands were assigned to free pigments (#0), antenna monomers (#1), LHCII trimers (#2), PSI/II cores (#3), and PSI/II supercomplexes (#4) according to Formighieri et al. (2012).