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. 2015 Dec 7;170(2):821–840. doi: 10.1104/pp.15.01458

Figure 3.

Figure 3.

Copurification of TEF30 with PSII particles. A, Nickel-affinity purification of PSII particles. Detergent-solubilized thylakoid membranes from a C. reinhardtii strain harboring a hexa-His-tagged D2 protein (D2His) and from wild-type control strain cc124 (WT) were subjected to nickel-affinity purification. Thylakoid membrane input proteins corresponding to 1 µg of chlorophyll and 4% of the imidazole eluate were separated by SDS-PAGE and immunodetected with antibodies against TEF30 and proteins representative for the major thylakoid membrane complexes. B, Silver staining of proteins immunoprecipitated from hexa-His-tagged PSII particles with preimmune serum (Pre) and antibodies against TEF30. C, Immunodetection of proteins immunoprecipitated from PSII particles. Purified PSII particles equivalent to approximately 1 µg of chlorophyll (Input) and 20% of the immunoprecipitates obtained with preimmune serum and antibodies against TEF30 (IP) were separated by SDS-PAGE and immunodecorated with the antisera indicated.