Figure 6.
Comparison of LHCSR3 expression and oxygen evolution in control and TEF30-amiRNA cells. A, Time course of LHCSR3 accumulation at 600 μmol photons m–2 s–1 (HL) in cw15-325 control (Con) and TEF30-amiRNA cells grown in Tris-acetate-phosphate (TAP)-NH4, TAP-NO3, or Tris-minimal-phosphate (TMP)-NH4. Whole-cell proteins were separated by SDS-PAGE and immunodetected with antibodies against LHCSR3 and against CF1β as loading control. B, Oxygen evolution from thylakoid membranes isolated from cw15-325 control and TEF30-amiRNA cells grown in TAP-NH4 medium at LL of approximately 30 µmol photons m−2 s−1 or HL of approximately 800 µmol photons m−2 s−1 for 4 h. Values represent means from experiments on two independent control and TEF30-amiRNA lines, and error bars indicate sd. (Note that the same thylakoid preparation was used for all PFDs applied, thus explaining the decline in oxygen evolution at 1,600 µmol photons m−2 s−1.)