Figure 8.

PSII stability is not impaired in TEF30-amiRNA strains. A, TEF30-amiRNA and control (Con) cells in the cw15-325 background were grown at approximately 30 µmol photons m⁻² s⁻¹ in TAP medium in the presence or absence of 100 µg mL⁻¹ CAP for 12 h. Proteins from whole cells harvested during the time course corresponding to 0.5 or 1 µg of chlorophyll (depending on the antiserum used) were analyzed by immunoblotting. D1 levels were quantified with the FUSIONCapt Advance program. D1 levels were normalized to the values before the addition of CAP (at 0 h), and the ratio between D1 levels from TEF30-amiRNA strains and controls was calculated. Values represent means from four independent replicates, and error bars indicate sd. B, TEF30-amiRNA and control cells in the cw15-325 background were grown in TMP minimal medium at 200 μmol photons m^–2 s^–1 in the presence of 100 µg mL⁻¹ CAP for 12 h. Analyses were done as in A, with two biological replicates. C, TEF30-amiRNA and control cells in the cw15-325 background were grown in TAP medium in the dark in the presence of 100 µg mL⁻¹ CAP for 72 h. Analyses were done as in A, with two biological replicates.