Figure 9.

Not PSII sensitivity to HL, but PSII repair, is impaired in TEF30-amiRNA strains. A, Cells of cw15-325 control (Con) and TEF30-amiRNA strains were photoinhibited (PI) for 60 min at 1,800 μmol photons m^–2 s^–1 in the presence of the chloroplast translation inhibitors CAP and lincomycin and allowed to recover at 30 μmol photons m^–2 s^–1 (LL) without inhibitors. PSII fluorescence was measured by pulse amplitude-modulated fluorometry. Values represent means from two biological replicates, and error bars indicate sd. B, Immunoblot analysis of whole-cell proteins collected from the experiments described in A. Results from a typical experiment are shown at the top, and means from quantified D1 signals (normalized relative to the initial D1 signal) are shown at the bottom. Values represent means from five biological replicates, and error bars indicate sd.