Figure 2.

TALEN-mediated mutation frequency at the waxy locus summarized by mutation type in wild-type (cv Dongjin) and oslig4-1 mutant calli. Box plots show substitution (A); insertion, including the combination of insertion with deletion (B); deletion (C); and total (D) mutation frequency, determined as the number of mutation reads per number of total reads. Boxes indicate the range of the 25th to 75th quartiles of the data, and the horizontal lines in the boxes show the median value for each genetic background. Vertical lines represent the maximum and minimum of all of the data. +/−, Heterozygous oslig4-1 mutants; −/−, homozygous oslig4-1 mutants. The wild type (cv Dongjin), n = 10; heterozygous oslig4-1 mutants, n = 11; homozygous oslig4-1 mutants, n = 20.