Table I. Mutation types and frequencies in the waxy locus in Pubi:wx_L1/R2 TALEN-expressing cv Nipponbare calli.
The spacer region between the two TALEN binding sites is shown in lowercase letters. Deletions are shown as hyphens, and other types of mutations are shown in bold. d#, Number of bases deleted; s#, number of bases substituted.
| Line No. | Total No. of Clones Analyzed | Sequence | Mutation Type | No. of Mutant Clones | Frequency of Mutations |
|---|---|---|---|---|---|
| % | |||||
| Wild type | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | ||||
| 3 | 48 | TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 | 4 | 8.33 |
| 11 | 48 | TCCTTATAAGCACATATggcatt-taatatatATGTTTGAGTTTTAGCGACA | d1 | 1 | 10.42 |
| TCCTTATAAGCACATAT-------------atATGTTTGAGTTTTAGCGACA | d13 | 1 | |||
| TCCTTATAAGCA---------------------------AGTTTTAGCGACA | d27 | 1 | |||
| TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 | 1 | |||
| TCCTTATAAGCACATATgg---------------------53-bp deletion---- | d53 | 1 | |||
| 12 | 48 | TCCTTATAAGCACATATggcat---aatatatATGTTTGAGTTTTAGCGACA | d3 | 1 | 4.17 |
| TCCTTATAAGCACATAT-----------atatATGTTTGAGTTTTAGCGACA | d11 | 1 | |||
| 14 | 46 | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 | 14 | 30.43 |
| 15 | 48 | TCCTTATAAGCACATATggcat------------GTTTGAGTTTTAGCGACA | d12 | 1 | 14.58 |
| TCCTTATAAGCACATATagcattgtaatatatATGTTTGAGTTTTAGCGACA | s1 | 1 | |||
| TCCTTATAAGCACATATggcatt-taatatatATGTTTGAGTTTTAGCGACA | d1 | 2 | |||
| TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 | 1 | |||
| TCCTTATAAGCACATATggcat------atatATGTTTGAGTTTTAGCGACA | d6 | 1 | |||
| TCCTTATAAGCACATATggcat--------atATGTTTGAGTTTTAGCGACA | d8 | 1 | |||
| 36 | 48 | TCCTTATAAGCACATATggcat-ataatatatATGTTTGAGTTTTAGCGACA | d1s1 | 4 | 16.67 |
| TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 | 4 |