Table II. Mutation patterns in the waxy locus in Pubi:wx_L1/R2 TALEN-expressing T0 regenerated cv Nipponbare plants.
The spacer region between two TALEN binding sites is shown in lowercase letters. Deletions are shown as hyphens, and other types of mutations are shown in bold. d#, Number of bases deleted; i#, number of bases inserted; wt, wild-type sequence.
| Line No. | Zygosity | Sequence | Mutation Type |
|---|---|---|---|
| Wild type | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | ||
| 12-2 | Monoallelic | TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 |
| 12-4 | Monoallelic | TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 |
| 12-5 | Biallelic | TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 |
| TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 | ||
| 12-8 | Monoallelic | TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 |
| 12-11 | Monoallelic | TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 |
| 14-8 | Monoallelic | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 |
| 14-10 | Monoallelic | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 |
| 14-11 | Monoallelic | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 |
| 15-39 | Monoallelic | TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 |
| 15-40 | Monoallelic | TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 |
| 15-43 | Monoallelic | TCCTTATAAGCACATATgg------------------TCCTTTTTAGCGACA | d18i4 |
| 36-2 | Monoallelic | TCCTTATAAGCACATATggcat------atatATGTTTGAGTTTTAGCGACA | d6 |
| 36-9 | Chimeric | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | wt |
| TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 | ||
| TCCTTATAAGCACATATggcat------atatATGTTTGAGTTTTAGCGACA | d6 | ||
| 36-12 | Chimeric | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | wt |
| TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 | ||
| TCCTTATAAGCACATATggcattg---------------AGTTTTAGCGACA | d15 | ||
| TCCTTATAAGCACATATggcat----------ATGTTTGAGTTTTAGCGACA | d10 | ||
| ----------28 bp-------------atatATGTTTGAGTTTTAGCGACA | d28 | ||
| 36-20 | Monoallelic | TCCTTATAAGCACATATggcatt--aatatatATGTTTGAGTTTTAGCGACA | d2 |
| 36-26 | Monoallelic | TCCT-------------------cagagatatATGTTTGAGTTTTAGCGACA | d19i5 |
| 36-32 | Chimeric | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | wt |
| TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 | ||
| TCCTTATAAGCACATATggcat----atatatATGTTTGAGTTTTAGCGACA | d4 |