Table III. Germinal transmission of TALEN-induced mutations in the waxy locus from T0 plants to T1 progeny.
c, Chimeric mutation; d#, number of bases deleted; i#, number of bases inserted, wt, wild-type sequence.
| Line No. | T0 Plants |
Total No. of T1 Plants Analyzed | Mutation Segregation in T1 Progeny | |
|---|---|---|---|---|
| Zygosity | Target Genotype | |||
| 12-2 | Monoallelic | wt/i1 | 11 | wt/wt, 4; wt/i1, 4; i1/i1, 3 |
| 12-4 | Monoallelic | wt/d4 | 17 | wt/wt, 6; wt/d4, 7; d4/d4, 4 |
| 12-5 | Biallelic | d4/i1 | 16 | wt/d4, 1; d4/d4, 4; d4/i1, 8; wt/i1, 3 |
| 12-8 | Monoallelic | wt/i1 | 18 | wt/wt, 4; wt/i1, 10; i1/i1, 4 |
| 14-8 | Monoallelic | wt/d3 | 15 | wt/wt, 2; wt/d3, 8; d3/d3, 5 |
| 14-10 | Monoallelic | wt/d3 | 16 | wt/wt, 1; wt/d3, 10; d3/d3, 5 |
| 14-11 | Monoallelic | wt/d3 | 12 | wt/wt, 3; wt/d3, 5; d3/d3, 4 |
| 15-39 | Monoallelic | wt/d5 | 26 | wt/wt, 7; wt/d5, 12; d5/d5, 7 |
| 15-40 | Monoallelic | wt/d5 | 12 | wt/wt, 4; wt/d5, 5; d5/d5, 3 |
| 15-43 | Monoallelic | wt/d18i4 | 14 | wt/wt, 6; wt/d18i4, 4; d18i4/d18i4, 4 |
| 36-2 | Monoallelic | wt/d6 | 14 | wt/wt, 5; d3/d6, 4; d3/d3, 4; c, 1 |
| 36-9 | Chimeric | c (wt/d5/d6) | 18 | wt/wt, 18 |
| 36-12 | Chimeric | c (wt/d4/d10/d15/d28) | 14 | wt/wt, 3; wt/d4, 9; d4/d4, 2 |