Table VI. Patterns of de novo mutation at the waxy locus depend on the dedifferentiation of Pubi:wx_L1/R2 transgenic T1 seeds.
The spacer region between the two TALEN binding sites is shown in lowercase letters. Mutations are shown in bold, and deletions are shown as hyphens. d#, Number of bases deleted; i#, number of bases inserted.
| Line No. | Generation | Sequence | Mutation Type |
|---|---|---|---|
| Wild type | TCCTTATAAGCACATATggcattgtaatatatATGTTTGAGTTTTAGCGACA | ||
| 12-8 | T0 plants | TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 |
| T1 plants | TCCTTATAAGCACATATggcattgtaaatatatATGTTTGAGTTTTAGCGACA | i1 | |
| T1 calli | TCCTTATAAGCACATATggcattg----atatATGTTTGAGTTTTAGCGACA | d4 | |
| TCCTTATAAGCACATATggcat------atatATGTTTGAGTTTTAGCGACA | d6 | ||
| TCCTTATAA------------------------------TTTTTTAGCGACA | d30 | ||
| TCCTTATAAGCACATATggcatt---86 bp--------------------- | d86 | ||
| TCCTTATAAGCACATATggcat--------atATGTTTGAGTTTTAGCGACA | d8 | ||
| TCCTTATAAGCACATATggcatttttatgaacgtttggtcctttttaTGAGTTTTAGCGACA | i24 | ||
| TCCTTATAAGCACATATgg---------tcatATGTTTGAGTTTTAGCGACA | d9i2 | ||
| TCCTTATAAGCACATAT---------------aaggagcAGTTTTAGCGACA | d15i7 | ||
| TCCTTATAAGCACATATggcat--------atATGTTTGAGTTTTAGCGACA | d8 | ||
| TCCTTATAAGCACATATggcat---aatatatATGTTTGAGTTTTAGCGACA | d3 | ||
| 14-8 | T0 plants | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 |
| T1 plants | TCCTTATAAGCACATATggcatt---atatatATGTTTGAGTTTTAGCGACA | d3 | |
| T1 calli | TCCTTATAAGCACATATggcatt-----atatATGTTTGAGTTTTAGCGACA | d5 |