Table VII. Frequency of TALEN-mediated mutations at the waxy locus in cv Dongjin (genetic background of oslig4-1), oslig4-1, cv Koshihikari (genetic background of oslig4-2), and oslig4-2 calli.
Mutation frequencies indicate the rate of calli bearing mutations per total calli analyzed by CelI assay. +/−, Heterozygous mutation; −/−, homozygous mutation.
| Experiment | Genotype | No. of Calli Analyzed | No. of Calli Bearing Mutations | Mutation Frequency |
|---|---|---|---|---|
| % | ||||
| Dongjin | 92 | 20 | 21.7 | |
| oslig4-1+/− | 94 | 19 | 20.2 | |
| oslig4-1−/− | 95 | 49 | 51.6 | |
| 1 | Koshihikari | 16 | 1 | 6.3 |
| oslig4-2−/− | 23 | 10 | 43.5 | |
| 2 | Koshihikari | 26 | 2 | 7.7 |
| oslig4-2−/− | 17 | 5 | 29.4 | |
| 3 | Koshihikari | 60 | 2 | 3.3 |
| oslig4-2−/− | 97 | 45 | 46.4 | |
| Total | Koshihikari | 102 | 5 | 4.9 |
| oslig4-2−/− | 137 | 60 | 43.8 |