Effect of Fe deficiency on sugar concentration in the roots of Col-0 in Arabidopsis. Five-week-old plants were grown in either complete (+ Fe) or Fe-free (-Fe) nutrient solutions. A, Concentrations of Suc, Fru (B), and Glc (C) in the roots were analyzed at 8, 12, 24, 48, and 96 h after each treatment. Data are expressed as mean ± sd (n = 4). An asterisk indicates significant differences between two treatments at each time point (one-way ANOVA, P < 0.05).