Figure 3.

Effects of dark treatment and exogenous Suc application on Fe deficiency responses in roots of Col-0 Arabidopsis. The 5-week-old plants were cultured in either complete (+ Fe) or Fe-free (- Fe) nutrient solutions. On day 3, a part of the plants was covered using a black box, and the above nutrient solutions were supplied with or without 2 mm Suc and the plants were continuously grown for 24 h. A, Relative FCR activity. B, Expression of FRO2. C, Expression of IRT1. Gene expression was analyzed by real-time qPCR. Transcript level of UBQ10 was used as an internal control. Data are means ± sd (n = 7). Different letters indicate significant differences among treatments (one-way ANOVA, P < 0.05).