Figure 4.

Comparison of Fe deficiency responses in roots between Col-0 plants and the suc2-5 mutants. The plants were precultured as described in “Materials and Methods.” Then the 20-d-old plants were transferred to either complete (+Fe) or Fe-free (- Fe) agar medium supplied with or without 2 mm Suc for 5 d. A, Relative FCR activity. B, Expression of FRO2. C, Expression of IRT1. Gene expression was analyzed by real-time qPCR. Transcript level of UBQ10 was used as an internal control. Data are means ± sd (n = 5-7). Different letters indicate significant differences among treatments with in a genotype (one-way ANOVA, P < 0.05). An asterisk shows a significant genotype by treatment interaction (two-way ANOVA, P < 0.05).