Figure 9.

Effects of exogenous Suc on Fe deficiency responses in roots of Col-0 plant treated with auxin transport inhibitor. The 5-week-old plants were cultured in either complete (+ Fe) or Fe-free (- Fe) nutrient solution with or without addition of auxin transport inhibitor NPA (5 μM). On day 3, the above nutrient solutions were supplied with or without 2 mm Suc, and the plants were continuously grown for 24 h. A, Relative FCR activity. B-D, Expression of FRO2, IRT1, and FIT. Gene expression was analyzed by real-time qPCR. Transcript level of UBQ10 was used as an internal control. Data are means ± sd (n = 5-7). Different letters indicate significant differences among treatments (one-way ANOVA, P < 0.05).