Figure 2.

In vitro transcription of Pol II-dependent genes with ANE-D. A, In vitro transcription assay from At U2, Nt GNPS gene, and Nt rbcS and Sl rbcS genes were compared with tobacco nuclear extracts (TNE) and ANE-D. At U2 gene was used as a positive control for Pol II activity. Expected extended products of reverse transcription from labeled primer are indicated by arrowheads, and α-amanitin tolerant signals are indicated by asterisks. B, Reaction conditions when α-amanitin (1 µg mL⁻¹) was added to the indicated concentrations. Reactions were performed with (+) or without (-) α-amanitin. RNA products were analyzed for an 8% (w/v) polyacrylamide / 8 m urea gel.