Esterification levels of glycerol OH groups of tomato fruit cutin isolated from the cus1-a mutant and Pro-35S:SlCUS1RNAi lines. A and C, Fruits at 20 DPA. B and D, Red ripe fruits. Left, cus1-a; right, Pro-35S:SlCUS1RNAi lines. WT, Wild type. Mean values ± sd were calculated from nine independent measurements (three fruits and three technical replicates each). Significant differences from the corresponding wild type are indicated by asterisks (Student’s t test; *, P < 0.05 and **, P < 0.01).