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. 2015 Dec 10;170(2):1090–1104. doi: 10.1104/pp.15.01649

Figure 2.

Figure 2.

THI1 overexpression increases the impact of ABA on stomatal closure and slow anion channel currents, and enhances drought tolerance. A, qPCR-based assessment of THI1 transcription in Col-0, and the overexpression lines 8 and 14 (THI1-OE-8 and THI1-OE-14). ACTIN2 was used as an internal control. B and C, ABA promotes stomatal closure more strongly in the overexpression lines than in the wild type. Shown: Photos (B) and width/length ratio analysis of the stomatal aperture (C). Error bars represent the se (n = 3). At least 60 stomata were measured for each genotype per replication. Bar in B = 10 µm. D, The rate of water loss from detached leaves. Data presented in the form mean ± se (n = 3). E, The drought response of the THI1 overexpression lines. F, The effect of supplying 50 µm ABA on slow anion currents in the guard cell protoplasts. G, Current/voltage relationships of whole cell slow anion currents as illustrated in F. The numbers of guard cells measured were: Col-0 (13), Col-0-ABA (11), THI1-OE-8 (9), THI1-OE-8-ABA (10), THI1-OE-14 (12), and THI1-OE-14-ABA (9). Data are shown in the form mean ± se. Asterisks in C, D, and G indicate significant differences between means (P < 0.05).