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. 2015 Dec 10;170(2):1090–1104. doi: 10.1104/pp.15.01649

Figure 4.

Figure 4.

The expression of CPK33 and the subcellular localization of CPK33. A to F, The behavior of plants harboring pCPK33::GUS. A, Ten-day-old seedling. B, Leaf. C, Guard cells. D, Root. E, Inflorescence. F, Silique. Bar in A, B, and F = 1 mm; in C = 10 μm; and in D and E = 0.5 mm. G to I, qPCR-based assessment of CPK33 induction in the presence of ABA (G), drought stress (H), and thiamine (I). Transcript levels relative to that of the reference gene ACTIN2 are presented, with each value being given in the form mean ± se (n = 3). Asterisks indicate significant differences between means (P < 0.05). J, The subcellular localization of p35S::CPK33-GFP expression in leaf protoplasts. K, Colocalization of GFP-tagged CPK33 and RFP-tagged THI1. To quantify the degree of overlap between two proteins, PSC colocalization analysis was performed. The values of fluorescence pixels across the two channels are depicted in an intensity scatter plot. rp, Linear Pearson correlation coefficient; rs, nonlinear Spearman’s rank correlation coefficient. L, The subcellular localization of pCPK33::CPK33-GFP expression in the root. Bars in J to L = 10 µm.