Table I. Summary of GT experiments targeting ALS locus.

TF First	TF Second Transformed Callia	BSR Calli	W548L, S627I Mutations in Endogenous ALS Gene					Ratio of Calli with W548L and S627I (%)b	Recovery of Regenerated Plantsc
			W548L and S627I		W548L		None
			Homo	Hetero	Homo	Hetero
Exp. 1
pCAMBIA-sGFP Mix	2753 (13.7g)	3	0	0	0	2	1	0	0
pZH_MMCas9 Mix	3994 (15.5g)	3	0	0	0	2	1	0	0
Exp. 2
pCAMBIA-sGFP no. 1	674	2	0	0	0	2	0	0	0
pCAMBIA-sGFP no. 10	665	0	0	0	0	0	0	0	0
pCAMBIA-sGFP no. 12	589	2	0	0	0	2	0	0	0
pZH_MMCas9 no. 1	821	4	0	0	0	2	2	0	0
pZH_MMCas9 no. 2	881	0	0	0	0	0	0	0	0
pZH_MMCas9 no. 3	830	1	0	0	0	1	0	0	0
Exp. 3
pZH_MMCas9 Mix	872	2	0	0	0	2	0	0	0
pZH_gLig4-1_MMCas9 Mix	985	19	0	8	0	7	4	0.812	4
pZH_gLig4-2_MMCas9 Mix	829	12	1	3	1	4	3	0.483	3
Exp. 4
pZH_MMCas9 no. 2	1109	0	0	0	0	0	0	0	0
pZH_MMCas9 no. 3	929	7	0	3	0	3	1	0.323	3
pZH_gLig4-1_MMCas9 no. 1	729	9	0	3	0	3	3	0.412	2
pZH_gLig4-1_MMCas9 no. 2	1100	24	3	8	1	8	4	1.000	7
pZH_gLig4-2_MMCas9 no. 5	682	5	0	1	0	2	2	0.147	1
pZH_gLig4-2_MMCas9 no. 10	1003	10	0	4	0	5	1	0.399	2
Exp. 5
T1 (lig4, +1(A) homo; pZH_gLig4-1_MMCas9, segregated out)	877	0	0	0	0	0	0	0	0

^aNumber of calli used for the transformation of GT vector, pPZ_mALS_g2&3.

^bGT (%) = (Number of calli with W548L and S627I mutations in endogeneous ALS gene/TF second transformed calli) × 100.

^cNumber of GT lines, in which regenerated plants were obtained.