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. 2015 Dec 23;170(2):1149–1161. doi: 10.1104/pp.15.01668

Figure 2.

Figure 2.

Membrane localization is crucial for the regulation of BR signaling in Arabidopsis by OsBSK3. A, The upper panel shows the G2A mutation. Red letters show the mutated amino acid. The middle and lower panels show the YFP signal detected by confocal microscopy in 1-week-old light-grown OsBSK3-YFP- and G2A-YFP-overexpressing bri1-5 leaves. B, 100 µg of soluble (S) or microsomal (M) proteins from OsBSK3-YFP- and G2A-YFP-overexpressing bri1-5 mutant plants were separated by SDS-PAGE and immunoblotted using anti-YFP antibodies. Coomassie Brilliant Blue (CBB) staining of the Rubisco large subunit was used as an equal loading control. C, Seven-week-old bri1-5 mutant plants overexpressing OsBSK3-YFP or G2A-YFP. G2A-1 and -8 are two independent transgenic lines. D, OsBSK3-YFP and G2A-YFP expression in the 3-week-old transgenic plants shown in C. Ponceau S staining of the Rubisco large subunit was used as an equal loading control.