Table I. The phosphorylated peptides identified from OsBSK3 and AtBSK3 by LC/MS/MS.
Peptidea | Measured [M+H]+ | Calculated [M+H]+ | Score | P-Value | Identified Sites |
---|---|---|---|---|---|
Identified in vitro phosphopeptides from OsBSK3 by CID | |||||
DGKpSYSTNLAFTPPEYM*R | 2172.9446 | 2172.9308 | 22.7 | 6.7E-06 | S213 |
DGKSYpSTNLAFTPPEYM*R | 2172.9389 | 2172.9308 | 34.8 | 2.1E-09 | S215 |
Identified in vivo phosphopeptides from OsBSK3 by HCD | |||||
pSYpSTNLAFTPPEYMR | 1856.7945 | 1856.7925 | 48 | 2.0E-11 | S213 or S215 |
Identified in vivo phosphopeptides from AtBSK3 by CID | |||||
pSYpSTNLAFTPPEYLR | 1838.8317 | 1838.8361 | 24.2 | 6.6E-06 | S210 or S212 |
Met sulfoxide is denoted as M*; phosphoseryl residues are denoted as pS.