Figure 1.

Theaflavin-3, 3′-digallate (TF3) inhibits OVCAR-3 cell-induced angiogenesis by targeting HIF-1α and VEGF. (A) Molecular structure of TF3. (B) Viability of OVCAR-3 cells was decreased after TF3 treatment for 24 h. (C) OVCAR-3 cells-induced HUVEC tube formation was inhibited by TF3 treatment. (D) OVCAR-3 cell-induced blood vessel development in the CAM model was reduced by TF3 treatment. (E) The protein level of VEGF in TF3-treated OVCAR-3 cell culture supernatant was reduced. (F) TF3 diminished the transcriptional activity of VEGF promoter, and, overexpression of HIF-1α reversed it. The data are presented as the mean ± standard error of mean. ^*P<0.05 compared with control or between specific groups.